Enzyme entrapment, biocatalyst immobilization without covalent attachment

The biological role of enzyme attachment and immobilization.

The state of enzymes in the living cell is considered: it is not clear whether some, if any, enzymes are naturally present in the "soluble" state. The evidence for enzyme binding to cellular constituents, and its effects on function and properties of enzyme and isoenzymes, is reviewed.

Site-Directed Covalent Immobilization

Covalent Immobilization of Native Biomolecules

We have worked out a procedure for covalent binding of native biomacromolecules on flat gold surfaces for scanning probe microscopy in aqueous buffer solutions and for other nanotechnological applications, such as the direct measurement of interaction forces between immobilized macromolecules, of their elastomechanical properties, etc. It is based on the covalent immobilization of amino group-c...

Stabilization of Penicillin G Acylase by Immobilization on Glutaraldehyde-activated Chitosan

The objective of this work was to study enzyme immobilization on chitosan activated with glutaraldehyde, aiming to produce a cheap biocatalyst. Two different immobilization strategies were studied: one-point and multipoint covalent attachment to the solid matrix. The multipoint covalent attachment derivative had an 82% immobilization yield. It was 4.9-fold more stable than the free enzyme at 50...

Improvements of enzyme activity and enantioselectivity via combined substrate engineering and covalent immobilization.

Esterases, lipases, and serine proteases have been applied as versatile biocatalysts for preparing a variety of chiral compounds in industry via the kinetic resolution of their racemates. In order to meet this requirement, three approaches of enzyme engineering, medium engineering, and substrate engineering are exploited to improve the enzyme activity and enantioselectivity. With the hydrolysis...